Open Access Original Research Article

Phytochemical Screening and Determination of Polyphenols in the Hydro-Ethanolic Extract of Trunk Bark and Its Fractions of Stereospermum kunthianium Cham (Bignoniaceae)

Abdou Sarr, Serigne Ibra Mbacké Dieng, Kady Diatta-Badji, Amadou Ibrahima Mbaye, William Diatta, Awa Ka, Alioune Dior Fall, Emmanuel Bassène

Asian Plant Research Journal, Page 1-9
DOI: 10.9734/aprj/2021/v7i130142

Background : Stereospermum kunthianum Cham., is a medicinal plant from the Bignoniaceae family, used in Ferlo (Senegal) against stomach aches, as a healing agent and aphrodisiac.
Aims/Objective : The aim of this study was to contribute to the valorisation of the plant by carrying out a phytochemical screening followed by an assay of the polyphenols of the hydro-ethanolic extract of the bark and its fractions.
Methods : Phytochemical screening was carried out by coloring and/or precipitation reactions. The total polyphenol and tannin contents were evaluated by the Folin-Denis method and the flavonoid content by a method using aluminium chloride (AlCl 3 ) and sodium nitrite (NaNO 2 ).

Results: The search for chemical compounds revealed the presence of various secondary metabolites such as gallic and catechic tannins, flavonoids, saponosides, alkaloids, sterols and polyterpenes. The total polyphenol contents of the samples ranged from 0.81 ± 0.02 to 13.52 ± 0.06 mg EAT / g. Those of tannins and flavonoids were between 0.03 ± 0.01 and 4.56 ± 0.02 mg EAT / g and between 1.13 ± 0.04 to 31.88 ± 0.19 mg ER / g respectively.
Conclusion : These different metabolites would be responsible for the activities noticed for this plant.

Open Access Original Research Article

Effect of Planting Density on Yield Components of False Flax

Eray Tulukcu

Asian Plant Research Journal, Page 10-15
DOI: 10.9734/aprj/2021/v7i130144

This research was carried out to determine the effect of population density on the performance components of false flax (Camelina sativa (L.) Crantz). This would allow to determine the effect of increasing the number of seeds of the false flaxon the yield and also the appropriate sowing distance under Konya conditions. The research was developed in the experimental field of Çumra Vocational School, Konya, Turkey. The experimental design was randomized complete blocks designwith three repetitions. Analyzes were made using the Jump statistics program.A thousand seeds were weighed and 4 different planting population densities were used: 500, 750, 1000 and 1250 seeds per square meter.On May 2, 2014 it was sown and on August 10, 2014 it was harvested. Plant height, number of plants per m2, biomass, root length, number of capsules, number of seeds per capsules, weight of a thousand seeds and other yield values were evaluated. The vegetative period of false flax under Konya ecological conditions was 100 days. The thousand-grain weight was 1.4 g (500) to 2 g (1000), plant height 58.3 cm (500) to 74 cm (750) and root length 9.6 cm (1000) to 16.3 cm (750). The number of capsules per plant and of seeds per capsule ranged from 152.6 (750) to 571.6 (500) and from 11 (1000) to 19.6 (500). In this study, It was found that  applications of 1250 seeds per m2 were the most suitable sowing density for yield

Open Access Original Research Article

Eco-physiological Responses of Amaranthus cruentus L. to Deficit Irrigation under Different NPK 20:10:10 Fertilization Rates

Pascal Tabi Tabot, Mfombep Priscilla Mebong

Asian Plant Research Journal, Page 16-25
DOI: 10.9734/aprj/2021/v7i130145

The aim of this research was to investigate the responses of Amaranthus cruentus L. to deficit irrigation under fertilization, in a 2 by 3 factorial experiment with two levels of irrigation (1.5 litre/week and 0.75 litre/week) corresponding to 2600 and 1300 mm/year respectively and three levels of NPK 20:10:10 (0, 138, 275 kg ha-1). This experiment was conducted in a screen house in Cameroon, and lasted for 12 weeks after the nursery phase. Growth parameters and chlorophyll fluorescence were measured weekly for 8 weeks. Destructive sampling was done at 12 WAT to determine biomass partitioning, water use efficiency and the root/shoot ratio. Data were analyzed for variance and relationships in the MINITAB Version 17 statistical package. Within each irrigation level, plant mass decreased as fertilizer rates decreased, while root: Shoot ratio increased instead. Plant mass expressed higher values at the higher irrigation levels while root: Shoot ratio was lower compared to that at lower irrigation levels. This shows a strategy for resource re-allocation to roots under both water and nutrient deficit. Harvest index was statistically similar across irrigation and fertilizer levels. Within the higher irrigation levels, WUE of plants decreased with a decrease in fertilizer rates but not for plants subjected to deficit irrigation. While chlorophyll fluorescence values differed significantly across treatments, all values were below 0.8, indicative of stress. Factor analysis showed that growth of A. cruentus was highly fertilizer-dependent, while chlorophyll fluorescence was irrigation-dependent. This suggest that fertilizer application is essential in ameliorating the effects of deficit irrigation, and will be essential in the production of this crop under deficit irrigation.

Open Access Original Research Article

Establishing of Optimum Nutrient Ranges for Canola Leaves Affected by Compost and Zinc by DRIS Analysis

Saied El Sayed, Farid Hellal, H. I. El-Aila

Asian Plant Research Journal, Page 26-35
DOI: 10.9734/aprj/2021/v7i130146

DRIS method is one of the important methods that reflect the status of the nutrients within the plant tissue. Field experiment were carried out at the Research and Production Station of the National Research Centre, Nubaria region, Beheira Governorate, Egypt during 2019/2020 to evaluate leaf nutrient optimum ranges of canola affected by compost addition (0, 2, 4 and 6 ton fed-1) to the soil and foliar application of zinc (0, 50 and 100 ppm) under water regimes at 75 and 40% water holding capacity. A remarkable increase the macronutrient contents due to the addition of compost and had a positive effect, especially with the rates 4 and 6 ton fed-1 + zinc sulfate foliar spraying at a rate of 100 ppm under sufficient and deficit irrigation treatments. Under water stress, in the control treatment as compared to the treatment combination (4 ton/fed compost + 100 ppm Zn), the N index was decreased from (-143.2) to (-76.1) in this time the nitrogen a negative DRIS index indicates that the nutrient level is below the optimum. Phosphorus index was recorded (-98) reduced to (-39.4) and a negative DRIS index indicates that the phosphorus level is below optimum in these treatment combinations. Also, the DRIS index for potassium was recorded (241.2) decrease up to (115.5) and the potassium has a positive DRIS index indicates that the nutrient level is above or near to the optimum. DRIS norms could be used to test the nutritional balance of nutrients in plant and diagnose nutrient requirements through calculating DRIS indices or direct application of physiological diagnosis (PD) chart. 

Objective: The objectives this study to evaluation of canola leaf nutrient optimum ranges affected by organic compost addition to the soil and micronutrient such as of zinc foliar application.

Open Access Original Research Article

GC-MS Analysis of Chemical Constituents of Hydroalcoholic Leaf Extract of Cissampelos Pareira and Their Anti-Diabetic Activity

Mohammad Asif, Sadaf Jamal Gilani, Mohamad Taleuzzaman, Chandra Kala, Deepak Godara, Iqra Rahat, Najam Ali Khan

Asian Plant Research Journal, Page 36-49
DOI: 10.9734/aprj/2021/v7i130148

Aim: The present work deals with the GC-MS-analysis of chemical constituents of hydroalcoholic extract of Cissampelos pareira leaves and thier anti-diabetic activity.

Methods: GC-MS analysis of extract was performed using Shimadzu QP-2010 plus with thermal desorption system 20. Acute oral toxicity of extract was done using the Organization of Economic Co-operation and Development (OECD) guideline 423. Diabetes was induced by single dose of streptozotocin 65 mg/kg, i.p. to all the rats except in rats of control group. Following which oral glucose tolerance test was performed and the rats were divided into various experimental groups. Various treatments continued for 21 days. Parameters such as blood glucose level, body weight, liver enzymes, lipid profiles and oxidative markers were checked.

Results: GC-MS analysis of the extract identified 25 compounds present in it. Based on acute oral toxicity study three doses of hydroalcoholic extract of Cissampelos pareira leaves viz 100, 200 and 400 mg/kg were selected for evaluation of anti-diabetic activity. The extracts at doses 200 and 400 mg/kg BW were able to reduce blood sugar level, liver enzymes, total cholesterol, total triglyceride, low density lipoprotein and Malondialdehyde; and enhance body weight, high density lipoprotein and Glutathione significantly when compared to rats of negative control group. The effect of extract at dose 400 mg/kg was comparable to standard Glibenclamide.

Conclusion: Results conclude that the chemical constituents present in the hydroalcoholic extract of Cissampelos pareira contained some anti-diabetic compounds possessing strong anti-diabetic activity.