Anatomical Studies of the Foliar Epidermis and Petiole of some Lannea Species in Jos, Plateau State, Nigeria
Asian Plant Research Journal,
Aims: Leaf epidermal morphology and petiole anatomy of seven Lannea species namely; Lannea velutina, Lannea kerstingii, Lannea egregia, Lannea schimperii, Lannea acida, Lannea microcarpa, and Lannea edulis in Nigeria were investigated to provide new taxonomic characters that could help in proper identification and delimitation of the taxa.
Place and Duration of Study: The study was carried out in the Department of Plant Science and Biotechnology, University of Jos, Nigeria between August 2020 and March 2021.
Methodology: Transverse sections of petioles were cut using a rotary microtome and epidermal peels were made. Staining was done using Safranin and slides observed using a light microscope.
Results: Stomatal distribution was hypostomatic except for L. velutina and L. edulis with amphistomatic distribution. Three stomata types are found; paracytic occurring in L. velutina, L. edulis and L. egregia, cyclocytic in L. schimperii and L. microcarpa and anomocytic in L. kerstingii and L. acida. Stomata index vary among taxa with L. egregia and L. acida having the highest (22.43%) and lowest (12.17%) respectively. The epidermal cell shape was polygonal in all the species and anticlinal wall patterns were straight to slightly curved. Unicellular/multicellular uniseriate trichomes were observed in L. schimperii, L. egregia and L. kerstingii distinguishing them from other species. Petiole outlines were circular to oval, epidermis was uniseriate and vascular bundles are collateral. Presence of continuous sclerenchyma rings surrounding the vascular bundles in L. kerstingii and L. microcarpa serve as a distinguishing character. Other distinguishing characters are presence of crystals, druses and phenolic idioblasts. A taxonomic key was produced using the anatomical character as an aid to the identification of the species.
Conclusion: Variations observed in the stomata types, petiole outline, petiole vasculature type, trichome type and stomata index could be employed for species identification and delimitation.
How to Cite
AbdulRahaman AA, Kolawole OS, Oladele FA. Leaf epidermal features as taxonomic characters in some Lannea species (Anacardiaceae) from Nigeria. Phytologia Balcanica. 2014;20(2-3):227-231.
Olowokudejo JD, Ayodele AE. The diversity of leaf epidermal features in the genus Hyptis Jacq.(Labiatae) in West Africa. Bio-Science Research Bulletin. 2007;23(2):141-158.
Kadiri AB, Oboh B, Oha C. systematic value of foliar epidermal morphology in some taxa of the tribes: Urticeae and Parietariae of the West African Urticaceae. Thaiszia Journal of Botany, Kosice. 2011; 21:73-83.
Olowokudejo JD. 1987. Taxonomic value of petiole anatomy in the genus Biscutella L. (Cruciferae). Bulletin du Jardin Botanique National de Belgique. 1987;57: 307-320
Akinnubi FM, Akinloye AJ, Oladipo OT. Petiole anatomy of some species of Asteraceae in Southwest Nigeria. African Journal of Plant Science. 2013;7(12):608-612.
Chhabra SC, Uiso FC, Mshiu EN. Phytochemical screening of Tanzanian medicinal plants. International Journal of Ethnopharmacology. 1987;11:157-179.
Ellenberg H, Weber HE, Du R, Wirth V, Werner W, Paulissen D. Ecological investigation in forest island in the Gambia. Vol 2. Gambia: Gambia Press; 1998.
Asase A, Oteng-Yeboah AA, Odamtten GT, Simmonds MS. Ethnobotanical study of some Ghanaian anti-malarial plants. Journal of Ethnopharmacology. 2005;99: 273-279.
Rafiu BO, Sonibare AM, Adesanya EO. Phytochemical screening, antimicrobial and antioxidant studies of Lannea egregia Engl. and K. Krause (Anacardiaceae) stem bark. Journal of Medicinal Plants Economic Development. 2019;3(1):2-9.
Faleyimu OI. Indigenous uses of medicinal plants for the treatment of farm animals in Rafi Local Government Area, Niger state, Nigeria. Journal of Sustainable Development in Africa. 2015;17:1-11.
Djoueche CM, azebaze AB, Dongmo AB. Investigation of plants used for the ethnoveterinary control of gastrointestinal parasites in Benoue region, Cameroon. Tropiculture. 2011;29:205-211.
Burkill HM. The useful plants of West Tropical Africa. Kew: Royal Botanic Gardens; 1995.
Elamin EE. Anatomical structure of three species of Lannea. Acta Scientific Agriculture. 2018;21(4):2-4.
Metcalfe CR, Chalk L. Anatomy of the Dicotyledons: Systematic Anatomy of Leaf and Stem, with a Brief History of the Subject. 2nd Edition. Oxford: Clarendon Press; 1979.
Stace CA. Cuticular studies as an aid to plant taxonomy. Bulletin of British Museum (Natural History) Botany.1965;4:3-78.
Johansen DA. Plant microtechnique. New York: McGraw-Hill; 1940
Fahn A. Plant Anatomy. 2nd edition. Oxford: Pergamon Press; 1997.
Soladoye MO. Leaf epidermal studies in the African genus Baphia Lodd. and related genera (Papilionoideae-Sophoreae). Bulletin du Jardin Botanique National de Belgique. 1982;52:415-437
Aworinde DO, Ogundairo BO, Osuntoyinbo KF, Olanloye OA. Foliar epidermal characters of some Sterculiaceae species in Nigeria. Bayero Journal of Pure and Applied Sciences. 2012;5(1):48-56.
Chukwuma EC, Soyewo LT, Okanlawon TF, Ugbogu OA. Foliar and petiole anatomy of Pterygota (Sterculioideae; Malvaceae) species and their distribution in Nigeria. Anales de Biología. 2017;39: 103-109.
Nurul-Aini CAC, Noraini T, Latiff A, Chung RCK, Nurhanim MN, Ruzi M. Systematic significance of petiole anatomical characteristics in Microcos L. (Malvaceae: Grewioideae). Malayan Nature Journal. 2013;65:145-170.
Santos RF, Nunes BM, Sá RD, Soares LAL, Randau KP. Morpho-anatomical study of Ageratum conyzoides. Revista Brasileira de Farmacognosia. 2016;26: 679-687.
Noraini T, Ruzi AR, Ismai BS, Ummu-Hani lB, Salwa S, Azi-Azeyanty J. Petiole vascular bundles and its taxonomic value in the tribe dipterocarpeae (Dipterocarpaceae). Sains Malaysiana. 2016;45:247-253.
Ogunkunle ATJ, Oladele FA. Leaf epidermal studies in some Nigerian species of Ficus L. (Moraceae). Plant Systematic Evolution. 2008;274:209-221
AbdulRahaman AA, Oladele FA. Leaf micromorphology of some Amaranthus species. Nigerian Journal of Pure and Applied Science. 2010;23:2136-2143.
Davis PA, Heywood VH. Principle of angiosperm taxonomy. Edinburgh: Oliver and Boyd; 1963.
Adedeji O, Jewoola DA. Importance of leaf epidermal characters in the Asteraceae family. Notulae Botanicae.Horti, Agrobotanic cluj-Napoca. 2008;36(2):7-16.
Essiett UA, Akpabio KE. The comparative anatomy of Talinum triangulare and Talinum portulacifolium in Nigeria. International Journal of Biotechnology and Allied Science. 2009;4(1):424-432.
Huan-Fang L, Yun-Fei D, Jing-Ping L. Foliar trichomes of Croton L. (Euphorbiaceae: Crotonoideae) from China and Its taxonomic implications. Bangladesh Journal of Plants Taxon. 2013;20(1):85-94.
Ogundipe OT, Olatunji K. Vegetative anatomy of Brachiaria obtussiflora and B. callopus. Feddes Report. 1991;102:159-165.
Adedeji O, Illoh HC. Comparative foliar anatomy of ten species in the genus Hibiscus Linn.in Nigeria. New Botanist. 2004;31:147-180.
Sharma BG, Albert S, Dhaduk HK. Petiolar anatomy as an aid to the Identification of Mangifera indica L. Varieties. Notulae Scientia Biologicae. 2012;4(1):44-47.
Cahyanto T, Sopian A, Efendi M, Kinasih I. The diversity of Mangifera indica cultivars in Sabang West Java based on morphological and anatomical characteristics. Biosaintifika. 2017;9(1): 156-167.
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