In-vitro Anti-bacterial Activity of Methanol and Aqueous Crude Extracts of Horsfieldia iryaghedhi

RMHKK Rajapaksha

Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Werahera, Sri Lanka.

EMN Fernando

Department of Pharmacy, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Sri Lanka.

AWMKK Bandara

Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Werahera, Sri Lanka.

NRM Nelumdeniya

Department of Pharmacy, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Sri Lanka.

ARN Silva *

Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Werahera, Sri Lanka.

*Author to whom correspondence should be addressed.


Abstract

Aims: Over the past two decades, the rise of multidrug resistance (MDR) in bacteria has posed a significant threat to global health. The urgent need for new treatment alternatives has brought attention to the potential of plants, which harbor a wealth of unexplored phytochemicals with therapeutic properties. This study aims to evaluate the anti-bacterial efficacy of methanol and aqueous extracts from the leaves and bark of Horsfieldia iryaghedhi In vitro.

Methodology: Aqueous and methanol extracts were obtained from the cold maceration method. In vitro anti-bacterial activity of methanol and aqueous leaf, bark, and combination extracts were determined against gram-negative bacteria Escherichia coli (ATCC® 25922) and gram-positive bacteria Staphylococcus aureus (ATCC® 25923). The anti-bacterial assay for different concentrations of each extract was conducted through the well-diffusion method, with Gentamycin serving as the positive control.

Results: Methanol leaf and combination extracts of Horsfieldia iryaghedhi have shown a positive anti-bacterial response at their highest concentrations of 1000µg/mL and 500µg/mL against gram-positive bacteria Staphylococcus aureus while none of the extracts showed anti-bacterial activity against gram-negative E. coli at the experimented concentrations.

Conclusion: The study concludes that methanol extracts of H.iryaghedhi should be further analyzed for their anti-bacterial activity, and there could be potential lead molecules that can be developed as antibiotics.

Keywords: Horsfieldia iryaghedhi, anti-bacterial activity, Escherichia coli, Staphylococcus aureus


How to Cite

Rajapaksha, RMHKK, EMN Fernando, AWMKK Bandara, NRM Nelumdeniya, and ARN Silva. 2024. “In-Vitro Anti-Bacterial Activity of Methanol and Aqueous Crude Extracts of Horsfieldia Iryaghedhi”. Asian Plant Research Journal 12 (4):27-34. https://doi.org/10.9734/aprj/2024/v12i4259.

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