Phyto-constituents of the Dichloromethane Extracts of the Bulbs of Selected Genera in the Family Amaryllidaceae

Margaret Emmanuel Bassey *

Department of Botany and Ecological Studies, Faculty of Biological Sciences, University of Uyo, Akwa Ibom State, Nigeria.

Imoh Imeh Johnny

Department of Pharmaacognosy and Natural Medicine, Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria.

Ogechi Queeneth Iwu

Department of Botany and Ecological Studies, Faculty of Biological Sciences, University of Uyo, Akwa Ibom State, Nigeria.

Omodot Timothy Umoh

Department of Botany and Ecological Studies, Faculty of Biological Sciences, University of Uyo, Akwa Ibom State, Nigeria.

Augustine Ogenekevwe Ochuko

Department of Botany and Ecological Studies, Faculty of Biological Sciences, University of Uyo, Akwa Ibom State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

The analysis of phytoconstituents present in the dichloromethane extract of the bulb of Hippeastrum vittatum (L’Her.) Herb., Proiphys amboinensis (L.) Herb., Urceolina amazonica (LindenChristenh. & Byng., Hymenocallis littoralis (Jacq.) Salisb., Crinum jagus (J. Thomps.) Dandy and Zephyranthes carinata Herb. by Gas Chromatography-Mass Spectometry (GC-MS) analysis was carried out using standard methods. The Results of the GC-MS analysis of Hippeastrum vittatum, Proiphys amboinensis, Urceolina amazonica, Hymenocallis littoralis, Crinum jagus and Zephyranthes carinata revealed the presence of 50, 66, 61, 57, 56 and 61 peaks indicating the presence of the number of phytochemical constituents respectively. Phytol was present in Hymennocallis littoralis (0.41%) and Urceolina amazonica (0.79%) only. N-hexadecanoic acid, Di mevalonic acid lactone, Hexadecanoic acid methyl ester and Stigmasterol were present in all. Apiol and Xanthoxylin were absent in H. littoralis and P. amboniensis respectively. Oleic acid was present in Z. carinata (2.01%), P. amboniensis (1.85%) and U. amazonica (2.03%). Sakuranin was found in H. littoralis (3.42%), C. jagus (1.38%) and U. amazonica (4.43%). Linoleic acid ethyl ester was present in P. amboniensis (1.49%), U. amazonica (0.16%), H. littoralis (11.91%)  and Z. carinata (0. 8 8%).  The major components that delimited the species were as follows; In Hymenocallis littoralis was Liriodendromin (14.03%), Linoleic acid ethyl ester (11.91%) and Ethyl oleate (9.38%); in Zephranthes carinata; Phthalic acid, 4-methoxybenzyl methyl ester (15.34%) Gatanthamine, 3-O-acetyl-1, 2, dihydro (8.87%) Stigmasterol (5.87%); in Hippeastrum vittatum; N-benzyl-2-[1-(4-methoxyl-phenyl 1) – 1 H-tetrazo (14.94%),  Benzaminde, 2-amino-N-(4-ethoxyphenyl) (12.65%) and  1-(5-methyl-2-hydroxyphenyl)-3-phenyl propane (10.49%); in Crinum jagus; 2H-1-Benzopyran-7-ol, 3,4-dihydro-3-(2-hyrdo) (19.87%),  Benzamide, 2-amino-N- (4-ethoxyphenyl) (9.92%) and Stigmasterol (4.04%); in Proiphys amboniensis; Gamma-sitosterol (16.98%), Stigmasterol (11.49%) and N-Hexadecanoic acid(5.05%); in U. amazonica; 3-Furanacetic acid, 4-hexyl-2, 5-dihydro-2, 5 (12.73%), Benzamide, 2-amino-N- (4-ethoxyphenyl) (8.18%) and Phenyl, 2-(3,4-dihydro 2-methoxyl-2H-1-benzopy) (6.40%). The presence of various bioactive compounds may be responsible for the application of these species in the treatment and management of various ailments in folklore medicine. However, in vitro and in vivo studies, isolation of individual phytoconstituents and their mechanism of action may proceed to find a novel drug or lead compound for use as medicine.

Keywords: GC-MS analysis, phytochemical constituents, phytol, apiol, n-hexadecanoic acid, stigmasterol, oleic acid


How to Cite

Bassey , Margaret Emmanuel, Imoh Imeh Johnny, Ogechi Queeneth Iwu, Omodot Timothy Umoh, and Augustine Ogenekevwe Ochuko. 2024. “Phyto-Constituents of the Dichloromethane Extracts of the Bulbs of Selected Genera in the Family Amaryllidaceae”. Asian Plant Research Journal 12 (3):72-91. https://doi.org/10.9734/aprj/2024/v12i3255.

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