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Aims: The present study assessed the morphology of re-epithelized skin following experimental open wound in rat model treated with Vitellaria paradoxa oil.
Place and Duration of Study: Anatomy Department, Olabisi Onabano University, Ago Iwoye and Biochemistry Department, Ladoke Akintola University of Technology, Ogbomoso, Nigeria between June, 2018 and March, 2019.
Methodology: Excision wounds were surgically created on three groups of ten rats each (II-IV). Group I was assigned as normal control (no surgery), Group II was treated with Vitellaria paradoxa oil, Group III was treated with Dermazin and Group IV was treated with distilled water. Area (cm3) of the wound were measured on days 3, 6 and 9, the rate of wound healing, re-epithelization of the skin were assessed histologically while the thickness of the granular cells layer and the organization of the epidermal squamous cells were evaluated morphologically, the level of collagen was determined in the wounds treated with Vitellaria paradoxa oil and compared with the collagen level in wound treated with distilled water and dermazin.
Results: The wound areas significantly (P < 0.05) reduced in V. paradoxa oil treated group indicating the comparative efficacy of V. paradoxa oil. Histological observations indicated gradual and complete re-epithelization of the skin in Vitellaria paradoxa oil treatments group. Increased in collagen deposition in V. paradoxa oil treated group is also observed (P < 0.05).
Conclusion: The results of this morphology indicated that V. paradoxa oil is a good potential in re-epithelization of open wound with no clinical disadvantage such as body weight, rectal temperature, packed cell volume using rats as animal model.
Govindarajan RA, Pushpangadan P, Kumara B, Vijayakumar M. Ethnopharmacological approaches to wound healing Exploring medicinal plants of India. J Cell Sci. 2007;111:1929–1934.
McDonough AK, Curtis JR, Saag KG. The epidemiology of glucocorticoid-associated adverse events. Curr. Opin. Rheumatol. 2008;20:131–137.
Wild T, Rahbarnia A, Kellner M, Sobotka L, Eberlein T. Basics in nutrition and wound healing. Nutrition. 2010;26:862–866
Lodhi S, Singhai AK. Wound healing effect of flavonoid rich fraction and luteolin isolated from Martynia annua Linn. On streptozotocin induced diabetic rats. Asian Pac. J. Trop. Med. 2013;6:253–259.
Lovett P, Hag N. Evidence for a tropic selection of the Shea nut tree (Vitellaria paradoxa). Agro-forestry Systems. 2000; 48:293-304
Alfred T. "Fats and Fatty Oils". Ullmann's Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH. 2002.
Olaniyan AM, Oje K. Quality characteristics of shea butter recovered from shea kernel through dry extraction process. J. Food Sci Technol. 2007;44:404-407.
Goreja WG. Shea Butter: The Nourishing Properties of Africa's Best-Kept Natural Beauty. Amazing Herbs Press. New York, NY; 2004.
Kanwaljit KC, Ramon B, Rosalia A, Galina G, Anna NW. Shea butter contains no IgE-binding soluble proteins. Letter to the editor. J Allergy Clin Immunol. 2010;127 (3):680–682.
Tapiero H, Tew GN, Mathe G. Polyphenols: Do they play a role in the prevention of human pathologies?. Biomed. Pharmacother. 2002;56:200-207
Che CT, Wang ZJ, Chow MS, Lam CW. Herb-herb combination for therapeutic enhancement and advancement: Theory, practice and future perspectives. Molecules. 2013;18:5125–5141.
Akoma O, Nma N, Musa S, Salihu A. Nutritional and Phytochemical Composition of Vitellaria paradoxa (Shea Fruit Pulp). Int J Biochem Res Rev. 2018;22(1):1–7.
Tumen I, Suntar I, Keles H, Akkol EK. A therapeutic approach for wound healing by using essential oils of cupressus and juniperus species growing in Turkey. Evid Based Complement Alternat Med. 2012;7.
Kiernan JA. Histological and histochemical method (theory and practice), fourth edition. United Kingdom. Scion Publishing; 2008.
Braiman-Wiksman L, Solomonik I, Spira R, Tennenbaum T. Novel insights into wound healing sequence of events. Toxicol Pathol. 2007;35:767-779.
Aramwit P, Sangcakul A. The effects of sericin cream on wound healing in rats. Biosci, Biotechnol Biochem. 2007;71: 2473-2477.
Reinke JM, Sorg H. Wound repair and regeneration. European Surgical Research. 2012;49(1):35–43
Senapati AK, Giri RK, Panda DS, Satyanarayan S. Wound healing potential of Pterospermum acerifolium wild. with induction of tumor necrosis factor-
Kant V, Gopal A, Pathak NN, Kumar, Tandan SK, Kumar D. Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats,” International Immunopharmacology. 2014; 20(2):322–330
Singer AJ, Clark RA. Cutaneous wound healing. The New England Journal of Medicine. 1999;341:738-746.
Gilliver SC, Emmerson E, Bernhagen J, Hardman M J. MIF: A key player in cutaneous biology and wound healing. Experimental Dermatology. 2011;20(1):1–6.
Zhao R, Li QW, Li J, Zhang T. Protective effect of Lycium barbarum polysaccharide 4 on kidneys in streptozotocin- induced diabetic rats. Canadian Journal of Physiology and Pharmacology. 2009;87 (9):711–719
Moulin V, Auger FA, Garel D, Germain L. Role of wound healing myofibroblasts on re-epithelization of human skin. Burns. 2000;26: 3.
Clark RAF. Wound repair: Overview and general considerations. In: CLARK RA AND HENSON PM. The molecular and cellular biology of wound repair, 2nd Ed, New York: Plenum. 1996;3-50.
Kondo T. Timing of skin wounds. Leg Med. 2007;9:109-114.
Shimizu N., Watanabe T, Arakawa T, Fujiwara Y, Higuchi K, Kuroki T. Pentoxifylline accelerates gastric ulcer healing in rats: Roles of tumor necrosis factor alpha and neutrophils during the early phase of ulcer healing. Digestion 2000;61:157-164.
Silvam JP, Rodarte RS, Cal heiros AS, Souza CZ, Amen doeira FC, Martins MA, Silva PM, Frutuoso VS, Barreto E. Antinociceptive activity of aqueous extract of Bowdichia virgilioides in mice. J Med Food. 2010;13: 348-351.
Thomazzi SM, Silva CB, Silveira DC, Vasconcellos CL, Lira AF, Cambui EV, Estevam CS, Antoniolli, AR. Antinociceptive and anti-inflammatory activities of Bowdichia virgilioides (sucupira). J Ethnopharmacol. 2010;127: 451-456.
Enoch S, Leaper D. Basic science of wound healing. Surgery. 2008;26:31–7.
Pattanayak SP, Sunita P. Wound healing, anti-microbial and antioxidant potential of Dendrophthoe falcata (L.f) Ettingsh. J Ethnopharmacol. 2008;120:241-7.