Optimization of IBA for Efficient Survival of Micropropagated Garcinia indica Choisy Shoots Using in-vitro and ex-vitro Rooting Techniques

Main Article Content

Gauri Mulik
Shubha Manvi
Gauri Ingale
Varsha A. Parasharami


Aims: The study focuses on optimization of concentration and time of rooting hormone exposure of IBA (Indole-3-Butyric Acid) for efficient survival of tissue culture raised Garcinia indica Choisy plantlets for in-vitro and ex-vitro rooting techniques.

Study Design: The subcultured microshoots of Garcinia indica were subjected to in-vitro and ex-vitro rooting trials by treating them with IBA of varying concentration and time, to standardize these particular parameters required by this auxin to induce rooting.

Place and Duration of Study: Rooting trials were carried out in Plant tissue culture-Biochemical Sciences Division of CSIR- National Chemical Laboratory, Pune 411008 between June 2018 and April 2019.

Methodology: Regularly subcultured five to six years old shoots from female trees of Garcinia indica were used for the study. Various concentrations of IBA in correlation with time were used for in-vitro and ex-vitro root induction. The rooted plantlets were then transferred to polyhouse for acclimatization and will further be planted in open field locations in June 2019.

Results: Induction of rooting was observed within thirty days of treatment with IBA. It was observed that 500ppm of IBA gave 30% rooting for in-vitro rooting trials whereas 2000ppm of IBA induced 80% rooting for shoots given ex-vitro rooting treatments. An interesting phenomenon that was observed for 70% of the shoots which failed to induce rooting by in-vitro treatment was that they survived with 100% rooting success under ex-vitro rooting conditions. The hardened plantlets were successfully acclimatized in the polyhouse with survival rate of 90% and were further transferred to polythene bags with rooting mixtures of sand: farmyard manure: coco peat (1:1:2). These plantlets have been healthy for the last 6 to 9 months and will be transported for field trials in June 2019.

Conclusion: Ex-vitro rooting technique was found to be more effective than in-vitro rooting. Thus, by optimizing the rooting hormone parameters, female plants of Garcinia indica can be successfully grown using tissue culture technology and can be propagated in large numbers to increase the female plant number in plantations.

Garcinia indica, IBA, optimization, in-vitro, ex-vitro, hardening

Article Details

How to Cite
Mulik, G., Manvi, S., Ingale, G., & Parasharami, V. (2019). Optimization of IBA for Efficient Survival of Micropropagated Garcinia indica Choisy Shoots Using in-vitro and ex-vitro Rooting Techniques. Asian Plant Research Journal, 2(4), 1-9. https://doi.org/10.9734/aprj/2019/v2i430050
Original Research Article


Parthasarathy U, Babu KN, Kumar RS, Ashis GR. Diversity of Indian Garcinia – A medicinally important spice crop in India. Acta horticulturae. 2013;979:467-476.

Jagtap P, Bhise K, Prakya V. A phyto-pharmacological review on Garcinia indica. International Journal of Herbal Medicine. 2015;3(4):2-7.

Kulkarni MD, Deodhar MA. In vitro regeneration and hydroxycitric acid productin in tissue culture of Garcinia indica Chois. Indian Journal of Bio-technology. 2002;1:301-304.

Chatterjee A, Yasmin T, Bagchi D, Stohs SJ. The bactericidal effects of Lactobacillus acidophilus, garcinol and Protykin compared to clarithromycin, on Helicobacter pylori. Molecular and Cellular Biochemistry Journal. 2003;243:29-35.

Elumalai A, Eswaraiah MC. A pharmaco-logical review on Garcinia indica Choisy. International Journal of Universal Pharmacy and Life Sciences. 2011;1:2249-6793.

Ramachandran HD. Plant profile, phytochemistry and pharmacology of Garcinia indica: A review. International Journal of Pharmaceutical Sciences. 2014; 27(2):376-381.

Thatte KS, Deodhar MA. Study of flowering behavior and sex determination in Garcinia indica (Thomas-Du Pettite) choisy by means of molecular markers biotechno-logy. 2012;11(4):232-237.

Chabukswar MM, Deodhar MA. Rooting and hardening of in vitro plantlets of Garcinia indica Chois. Indian Journal of Biotechnology. 2005;4:409-413.

Thengane SR, Deodhar SR, Bhaosale SV, Raval K. Repetitive Somatic Embryo-genesis and plant regeneration in Garcinia indica Choiss. In Vitro Cellular and Developmental Biology—Plant. 2006;42: 256-261.

Joshi PN, Hedge AN, Hedge VK. In-vitro propagation of Garcinia indica Chiosy from seedling explants. International Journal of Current Research. 2015;7:24676-24678.

Deodhar S, Pawar K, Singh N, Thengane RJ, Thengane SR. Clonal propagation of female plants of Garcinia indica Choiss: A tree species of high medicinal value. Journal of Applied Biology and Biotechno-logy. 2014;2:8-25.

Rajasekharan PE, Ganeshan S. Conservation of medicinal plant bio-diversity—An Indian perspective. Journal of Medicinal and Aromatic Plants. 2002; 24:132-147.

Parasharami V, Kunder G, Desai N. Recent pharmacological advances of endangered species of southern India: Garcinia indica Choisy. Journal of Scientific Research & Reports. 2015; 8(5):1-10.

Deb CR, Rout GR, Mao AA, Nandi SK, Singha RK, Vijayan D, Langhu T, Kikon ZP, Pradhan S, Tariq SM, Swain D. In vitro propagation of some threatened plant species of India. Current Science. 2018; 114:567-575.

Malik SK, Chaudhury R, Kalia RK. Rapid in vitro multiplication and conservation of Garcinia indica: A tropical medicinal tree species. Scientia Horticulturae. 2005;106: 539–553.

Deodhar SR, Thengane RJ, Thengane SR. De nova shoot regeneration from root cultures of Garcinia indica Chioss. Indian Journal of Experimental Biology. 2008; 46:482-486.

Lamaoui M, Chakchar A, Kharrassr YE, Wahbir S, Ferradous A, Mousabik AE. Selection and multiplication of Argan (Argania spinosa L.) Superior clones for conservation purposes. Acta Scientific Agriculture. 2019;3:116-123.

Panwar D, Patel AK, Shekhawat NS. An improvised shoot amplification and ex vitro rooting method for offsite propagation of Tinospora cordifolia (Willd.) Miers: A multi-valued medicinal climber. Indian Journal of Plant Physiology. 2018;1:169–178.

Jaime A, Teixeira D, Pacholczak A, Jlczuk A. Smoke tree (Coyinus coggygria Scop.) Propagation and biotechnology: A mini-review. South African Journal of Botany. 2018;114:232-240.

Chandra S, Bandopadhyay R, Kumar V, Chandra R. Acclimatization of tissue cultured plantlets: From laboratory to land. Biotechnol Lett. 2010;32:1199–1205.

Yan H, Liang C, Yang L. In vitro and ex vitro rooting of Siratia grosvenorii, a traditional medicinal plant. Acta Physiol Plant. 2010;32:115–120.

Ahmad Z, Shahzad A, Sharma S. Enhanced multiplication and improved ex vitro acclimatization of Decalepsis arayalpathra. Biologia Plantarum. 2018;1: 1-10.

Thatte KS, Deodhar MA. Study of flowering behavior and sex-determination in Garcinia indica (Thomas-Du Pettite) Choisy by means of molecular markers. Biotechno-logy, Asian network for scientific informa-tion. 2012;11(4):232-237.

Braganza M, Shirodhar A, Bhat DJ, Krishnan S. Resource Book of kokum (Garcinia indica Choisy). Western Ghats Kokum Foundation, Panji; Goa, India; 2012.